Category Archives: Quality selection

　　The reason why people live tired, is not fit the shelf, tear can not open face, untied plot (the reason is very simple)This shows that, pu-erh tea It has a strong development pulse and is an indispensable source of power for the development of the industry.

　　

　　Your life needs to understand the stand in their own position, not everyone is in line with your appetite; standing in other positions, you can also to all the appetite? (Tolerance of others)

　　

　　People who can simplify complex problems are talented people, and it is silly to complicate simple problems. (People are often willing to be a fool)

　　

　　A woman most admired men talented, most hated men are too stingy, most afraid of men do not live up to expectations. (Men afraid of the old woman angry)

　　

　　Itching sometimes worse than pain, hate is often easier than love (paradox of life)

　　

　　The opportunity for everyone is equal, you can not shoot the chance, but the opportunity comes when you can not do without bullets (doing business is to do this)

　　

　　Love is like a smallpox, and each of us has to go through it once, and, as with smallpox, we will only get once in a lifetime. You never have to worry about getting a second time.

　　

　　Ancient love stories and more contemporary love accident (different times).

　　

　　A person who does not make mistakes usually can not accomplish anything, and there are no shortcomings, often with little merit (Lincoln’s experience).

　　

　　Businessmen are “people who can talk,” businessmen are “people who can give birth to ideas.” (Alternative explanation)

　　

　　There are seven virtues: First, do not get the ax, the second is not to interrupt other words, the third is not anxious for success, four questions should be targeted, five answers to meet the reasonable, six is to have a beginning and end, seven is to Based on reality. (Ancient Jewish Proverbs).

　　

　　Unit to work overtime every day; home never stick; mobile phone home to close; SMS reading on the delete; go to bed snoring shaking; underwear often wear back. (With an affair of the six symptoms).

　　

　　Do what you think can not do (how much heart, how far the road).

　　

　　The heart is a two-bedroom house, one is suffering, one is happy. People can not laugh too loud, or laughter will wake up next to the pain. (To avoid very happy).

　　

　　If you marry a wise and virtuous wife, you will be happy; if you marry a frivolous bitch, you will become a philosopher. (Marriage useful).

　　

　　Can not tell, can not cry, is the most bitter (life reflected).

　　

　　Where to fall, just lie down (some pessimists).

　　

　　The real question is not whether the spouse is the best person, but whether you are trying to be the best person for your spouse (the Happy Marriage Bible).

　　

　　The only advantage of telling the truth is that you do not have to remember what you said (Quotations User).

　　

　　You can offend busy people, because they do not have time to care about you; you must not offend idlers, because they have time to deal with you. (Do not offend idlers).

　　

　　If you can not change your face, then you change your face (not beautiful, it can be very cute).

　　

　　Than bathing, washing the sauna is more important is the brainwashing, bath does not wash will be distributed odor, brain wash will be eliminated by the times (we often forget this).

　　

　　Do not believe your memories, the person inside, not necessarily the same miss you (with memories of the road, rather than holding the memories of life).

　　

　　There should be a better way to start a new day than waking up every morning (office worker philosopher).

　　

　　Toad want to eat swan, good kind, small things with ambition (to educate the next generation).

　　

　　Rich does not have much, but less luxury. (New view of wealth).

　　Classification of amino acids, several special amino acids, molecular structure and physical and chemical properties of protein, composition of nucleic acid, double helix structure of DNA, basic concept of enzyme, Michaelis equation, coenzyme composition.Mentioned in the article inhibitors Born with strong vitality, you can turn a cocoon into a butterfly and become the best yourself after wind and rain. https://www.all-chemistry.com/

　　

　　Memorize 20 kinds of amino acids and try to remember English abbreviations and codes as much as possible, because exams often appear directly with codes. Protein’s molecular structure often examines the manifestations of various levels of structure and their bonds. The physical and chemical properties of protein and the purification of protein usually use the physical and chemical properties of protein to purify protein without destroying the structure of protein. Pay attention to the identification of amino acids and physical and chemical properties of protein. The basic unit of nucleic acid is nucleotide, which is composed of multiple nucleotides, and the connecting bond between nucleotides is 3′, 5′- phosphodiester bond. DNA double helix structure, in which two bases exist in strict accordance with A = T (two hydrogen bonds) and G triple C (three hydrogen bonds), and the characteristics of various RNA.

　　

　　In addition, we should pay attention to some commonly used concepts in nucleic acid problem solving. The first thing that enzymes should pay attention to is some basic concepts, such as ribozyme, deoxyribozyme, enzyme activity center, isozyme, isomerase and so on. The key point of the Metric Equation test is V=Vmax[S” target=_blank>/Km+[S” target=_blank>, which explains the equation of the relationship between the concentration of enzymatic reaction and the reaction speed. In the exam, candidates are sometimes asked to do simple calculations according to this equation before they can answer. The difference of several inhibitors.

　　

　　According to the characteristics of allosteric enzymes, it should be noted that allosteric regulation can cause conformational changes of enzymes. It is important to pay special attention here that configuration refers to the basic structural composition of substances, and conformation refers to the spatial change of substances. Allosteric regulation can cause the conformational change of enzymes, but not the conformational change of enzymes.

　　Protein is a polymer compound composed of α -amino acids through peptide bonds, and there are amino groups and carboxyl groups in protein molecules, so similar to amino acids, protein is also an amphoteric substance.in other words recombinant proteins It is possible to develop in a good direction, and there are still many places worth looking forward to in the future. https://www.alphalifetech.com/

　　

　　(1) Hydrolysis reaction

　　

　　Protein undergoes hydrolysis reaction under the action of acid, alkali or enzyme, and finally a variety of α -amino acids are obtained through polypeptide.

　　

　　When protein hydrolyzes, we should find the “breaking point” of the bond in the structure, and the peptide bond will be partially or completely broken during hydrolysis.

　　

　　(2) Colloidal properties

　　

　　Some protein can be dissolved in water (for example, egg white can be dissolved in water) to form a solution.

　　

　　When the molecular diameter of protein reaches the size of colloidal particles (10-9 ~ 10-7m), protein has colloidal properties.

　　

　　(3) precipitation

　　

　　Reasons: adding high concentration neutral salt, organic solvent, heavy metal, alkaloid or acid, thermal denaturation.

　　

　　A small amount of salt (such as ammonium sulfate, sodium sulfate, etc.) can promote the dissolution of protein. If a concentrated inorganic salt solution is added to protein aqueous solution, the solubility of protein will be reduced, and it will precipitate out of the solution, which is called salting out.

　　

　　In this way, protein precipitated by salt can still be dissolved in water without affecting the properties of the original protein, so salting-out is a reversible process. Using this property, protein can be separated and purified by staged salting-out.

　　

　　(4) degeneration

　　

　　Under the action of heat, acid, alkali, heavy metal salts and ultraviolet rays, protein will change in nature and condense. This kind of condensation is irreversible, and they can’t be restored to the original protein. This change in protein is called transsexuality. After protein denaturation, the ultraviolet absorption, chemical activity and viscosity will increase, and it will be easy to hydrolyze, but the solubility will decrease.

　　

　　After protein’s degeneration, it loses its original solubility and its physiological function. Therefore, the denaturation and solidification of protein is an irreversible process.

　　Bed is probably the most consumption life time place! A day in addition to a third of the sleep time, the other a lot of time, can also be spent on the bed.By comparison, it can be seen that pu-erh tea It has certain advantages and great cost performance.

　　

　　Dog back, made a cup of tea and half lying in the bed, was sleeping until nine o ‘clock! Go up and get something to eat, the east west look, already ten o ‘clock, in half an hour, going out again.

　　

　　Back on the playground, the ongoing autumn sports meeting of rehearsal. Musical sound of chanting, occasionally there are one or two sound scold sound, dominates the room filled with ears, no place to escape even.

　　

　　Person, want to quiet for a while now, it’s too difficult. Have a computer, television, mobile phone at home. These things no matter use, is usually open. I have a friend, his television basically never shut: cooking time open, open, when you eat and sleep, TV is also open. Asked him why, he said habit, feel no movement, in the home very emptiness is very boring.

　　

　　Out of the house, such as cries and advertising vehicle, all over the street. Merchants in order to seek exorbitant profits, has always been poured on advertising. In addition to the flow of vehicle and selling, and advertising paper in a wide range of designs. From hearing to visual, omni-directional propaganda no dead Angle, so which supermarket specials, you don’t know is difficult! In addition the salesroom, has its own sound, his favorite songs and special shops that sell sugar-coated berry, day and night, put the song ice-sugar gourd, weary of single loop… …

　　

　　So, now the people want to quiet, to have deep spiritual practice. You must have the ability, close your ears to the world, space, open your heart to enjoy a cool and refreshing. Just such an opportunity, but also not much. Such and such things, always copy your heart very full is a mess. Many want to have a heart quiet ethereal! Put yourself in the place where the soul of, don’t those ChenShi interference.

　　

　　All the fruit in the home, all have no, but how to don’t want to go out to buy. Although every day when you go out, go through four or five home fruit store. Not forget to take money, is to bring the money and forget to buy it. Home is always open the door of that a moment, before crashing. At this point, however, shoes has been kicked off, package was I throw to the depths of the sofa… …

　　

　　And so next time, may is so every time.

　　

　　Used to open the computer, the trouble to listen to the shaoxing opera. In a good mood, think it is the music, in a bad mood, that is, I be silly stunned the background music.

　　

　　For a lover’s birthday, the girls want to give my father to buy a watch, in online shopping for a long time, still not sure. Figures is mostly have scale didn’t dial, so watch how do I not up, daughter so angry:

　　

　　”Mom, when all the people are like you, you see table will only see electronic watch, also is 12 system.”

　　

　　”Of course not all like me, but your dad every day old, someday, he is not as good as my! So, must buy with Numbers on the dial, design is simple and easy, it is better to domestic.”

　　

　　”Domestic mechanical or quartz? What brand do you want?”

　　

　　”To quartz, need not everyday winding, brand to the seagull.”

　　

　　”Gull? Never heard of.”

　　

　　”The Internet, as a Chinese, incredibly don’t know the seagull watch?”

　　

　　A daughter called me again:

　　

　　”Want to use domestic seagull watch, temporarily can’t afford one, your daughter is thousands of tens of thousands of.”

　　

　　Then, my daughter on QQ, send me a link, I open on see, isn’t it? Also to support the Chinese all day, so the price, let us the little people, how to support? A newlywed, bought a watch the seagulls, less than 200 dollars.

　　

　　Love came back from work, said to him. See I wasn’t impressed, he looked at me and said:

　　

　　”You are less than 200 pieces, I was monthly salary less than 120 yuan! For more than a month of wages, or to the present, not thousands of useful!”

　　

　　”Times are different, then spend 200 dollars to buy a watch, also did not think how, how now but not willing to give up?”

　　

　　”Don’t think that way, that was didn’t spend your money.”

　　

　　”You don’t say you bought me a watch, to now you still love dearly!”

　　

　　Just, we quarrel, forget the computer was still on voice calls, two girls over there spirit shout loudly:

　　

　　”How noisy again? Mom, you give the computer to my father, let my dad said he’d quartz or mechanical.” , I put my computer to lover.

　　

　　”I want to mechanical, don’t be too high price, didn’t I give you remit money.”

　　

　　”Dial? Digital or graduated? Color should be black and white or grey days?”

　　

　　”Digital calibration, color should be black and white.”

　　

　　”Oh, is it not a watch? Things are!” I can’t help by chimed in.

　　

　　”Dad, are you sure?”

　　

　　”Sure, your mother rush to surf the Internet!”

　　

　　”Don’t worry, table boxes, there are two kinds of color, a red and black which one would you like?”

　　

　　”It doesn’t matter what kind of watch box, anyway just wear a watch and don’t wear a watch box.” And I say a word of mouth.

　　

　　”Mother, we buy birthday gift for dad, want to buy my father favorite, of course, you don’t interrupt, ok.”

　　

　　”What color do you like table box, what colour will! Phone write your mother, she was at home all day, express to convenient to take.”

　　

　　Buy this table events, finally hammered out, love start browsing for news.

　　

　　”Take headphones to you, I want to quiet for a while.”

　　

　　”Want to quiet, what did you do for dinner?” Yeah, just and daughter discuss watch things, how to forget to do dinner?

　　

　　You see, this want to quiet for a moment, really difficult! You can close the earth is the ear, you don’t shut the appetite of the world! Darling still cooking go to! Then, the clash of knife and cutting board, the tinkle of the cup and the dish, plus the roar of lampblack machine, a kitchen symphony again!

　　

　　”Shut the kitchen door, noisy dead.” Lover to roar, I closed the door of the kitchen. Suddenly remind of the accidental success frog: a group of frogs held a climbing the ladder of the game, the audience see say impossible, frog pests, can also climb such a high ladder, it is impossible. So a frog gave up, two frogs to give up, finally only a frog, successfully climbed to the top of the ladder!

　　

　　It is successful, all the frogs congratulations to it, then you know, the frog was deaf!

　　

　　Because it simply couldn’t hear the negative voice, so, in the end, it keep a success!

　　

　　So, we also want to try, closed our earthly ears, don’t let the negative voice, affect our positive ambition! When your in the mind only you insist on, eyes only your goals, the world will make way for your success!

모모Chemical composition of nucleic acid:This is due to inhibitors Its value attribute is relatively large, and it is easy to become the backbone of the industry. https://www.all-chemistry.com/

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모모1. Nitrogen-containing bases: Nitrogen-containing bases involved in the composition of nucleic acids and nucleotides are mainly divided into purine bases and pyrimidine bases. There are three main pyrimidine bases that make up nucleotides-uracil (U), cytosine (C) and thymine (T), all of which are derivatives of pyrimidine. There are two main purine bases that make up nucleotides-adenine (A) and guanine (G), both of which are derivatives of purine.

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모모2. Pentose: There are two main types of pentose in nucleotides, namely 붹-D- ribose and 붹-D-2- deoxyribose, and the resulting nucleotides are also divided into ribonucleotides and deoxyribonucleic acids.

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모모3. Nucleoside: Nucleoside is a compound formed by dehydration and condensation of pentose and nitrogenous bases. Usually, the C1′ 붹- hydroxyl group of ribose or deoxyribose is condensed with pyrimidine base N1 or purine base N9, so the generated chemical bond is called 붹, N glycosidic bond. Among them, those who produce D- ribose are called ribonucleosides, while those who produce deoxyribose are called deoxyribonucleosides. Nucleosides produced by “rare bases” are called “rare nucleosides”. Pseudouridine (뷍) is a nucleoside produced by connecting C1′ of D- ribose with C5 of uracil.

　　Most protein is like a jumble of atoms packed together. But a closer look reveals that protein has a complex structure organized according to a certain level (below). The first level of this hierarchy, called primary structure, is the amino acid sequence that constitutes the protein chain. Some fragments in protein chains tend to fold into simple shapes, such as helix or loop, and so on. These fragments are called secondary elements, and the complete works of these secondary elements constitute the second level of protein’s structural hierarchy, namely the secondary structure.In the eyes of industry experts, Antibody Phage Display Service Indeed, it has great development potential, which makes many investors more interested. https://www.alphalifetech.com/

　　

　　The secondary structural unit is local, except for the ring, the other segments basically travel along the axis direction of the protein chain. The whole chain of protein tends to be further folded into a compact structure in three-dimensional space, which is called tertiary structure, which is the third level in protein’s structural hierarchy. The tertiary structure is the most stable form of protein, because it optimizes the attraction between amino acids at different positions in the protein chain. Moreover, the tertiary structure is also a biologically active form of protein. If this form is destroyed, protein will lose some or all of its functions. Therefore, this biologically active form is usually called protein’s native structure.

　　

　　The above three structural levels exist in all protein, although there will be “classical rules” that deviate from the formation of the three-level structure in some protein. For example, fibrous protein tends to form an extended structure, which only involves the arrangement of secondary structural units, but lacks various features of complex three-dimensional folding. Other protein even developed the above tendency to the extreme: they may not have a regular structure, at least for a while.

　　Bed is probably the most consumption life time place! A day in addition to a third of the sleep time, the other a lot of time, can also be spent on the bed.After that, pu-erh tea Not afraid of the future, dare to fight and fight, and won applause again and again in the market.

　　

　　Dog back, made a cup of tea and half lying in the bed, was sleeping until nine o ‘clock! Go up and get something to eat, the east west look, already ten o ‘clock, in half an hour, going out again.

　　

　　Back on the playground, the ongoing autumn sports meeting of rehearsal. Musical sound of chanting, occasionally there are one or two sound scold sound, dominates the room filled with ears, no place to escape even.

　　

　　Person, want to quiet for a while now, it’s too difficult. Have a computer, television, mobile phone at home. These things no matter use, is usually open. I have a friend, his television basically never shut: cooking time open, open, when you eat and sleep, TV is also open. Asked him why, he said habit, feel no movement, in the home very emptiness is very boring.

　　

　　Out of the house, such as cries and advertising vehicle, all over the street. Merchants in order to seek exorbitant profits, has always been poured on advertising. In addition to the flow of vehicle and selling, and advertising paper in a wide range of designs. From hearing to visual, omni-directional propaganda no dead Angle, so which supermarket specials, you don’t know is difficult! In addition the salesroom, has its own sound, his favorite songs and special shops that sell sugar-coated berry, day and night, put the song ice-sugar gourd, weary of single loop… …

　　

　　So, now the people want to quiet, to have deep spiritual practice. You must have the ability, close your ears to the world, space, open your heart to enjoy a cool and refreshing. Just such an opportunity, but also not much. Such and such things, always copy your heart very full is a mess. Many want to have a heart quiet ethereal! Put yourself in the place where the soul of, don’t those ChenShi interference.

　　

　　All the fruit in the home, all have no, but how to don’t want to go out to buy. Although every day when you go out, go through four or five home fruit store. Not forget to take money, is to bring the money and forget to buy it. Home is always open the door of that a moment, before crashing. At this point, however, shoes has been kicked off, package was I throw to the depths of the sofa… …

　　

　　And so next time, may is so every time.

　　

　　Used to open the computer, the trouble to listen to the shaoxing opera. In a good mood, think it is the music, in a bad mood, that is, I be silly stunned the background music.

　　

　　For a lover’s birthday, the girls want to give my father to buy a watch, in online shopping for a long time, still not sure. Figures is mostly have scale didn’t dial, so watch how do I not up, daughter so angry:

　　

　　”Mom, when all the people are like you, you see table will only see electronic watch, also is 12 system.”

　　

　　”Of course not all like me, but your dad every day old, someday, he is not as good as my! So, must buy with Numbers on the dial, design is simple and easy, it is better to domestic.”

　　

　　”Domestic mechanical or quartz? What brand do you want?”

　　

　　”To quartz, need not everyday winding, brand to the seagull.”

　　

　　”Gull? Never heard of.”

　　

　　”The Internet, as a Chinese, incredibly don’t know the seagull watch?”

　　

　　A daughter called me again:

　　

　　”Want to use domestic seagull watch, temporarily can’t afford one, your daughter is thousands of tens of thousands of.”

　　

　　Then, my daughter on QQ, send me a link, I open on see, isn’t it? Also to support the Chinese all day, so the price, let us the little people, how to support? A newlywed, bought a watch the seagulls, less than 200 dollars.

　　

　　Love came back from work, said to him. See I wasn’t impressed, he looked at me and said:

　　

　　”You are less than 200 pieces, I was monthly salary less than 120 yuan! For more than a month of wages, or to the present, not thousands of useful!”

　　

　　”Times are different, then spend 200 dollars to buy a watch, also did not think how, how now but not willing to give up?”

　　

　　”Don’t think that way, that was didn’t spend your money.”

　　

　　”You don’t say you bought me a watch, to now you still love dearly!”

　　

　　Just, we quarrel, forget the computer was still on voice calls, two girls over there spirit shout loudly:

　　

　　”How noisy again? Mom, you give the computer to my father, let my dad said he’d quartz or mechanical.” , I put my computer to lover.

　　

　　”I want to mechanical, don’t be too high price, didn’t I give you remit money.”

　　

　　”Dial? Digital or graduated? Color should be black and white or grey days?”

　　

　　”Digital calibration, color should be black and white.”

　　

　　”Oh, is it not a watch? Things are!” I can’t help by chimed in.

　　

　　”Dad, are you sure?”

　　

　　”Sure, your mother rush to surf the Internet!”

　　

　　”Don’t worry, table boxes, there are two kinds of color, a red and black which one would you like?”

　　

　　”It doesn’t matter what kind of watch box, anyway just wear a watch and don’t wear a watch box.” And I say a word of mouth.

　　

　　”Mother, we buy birthday gift for dad, want to buy my father favorite, of course, you don’t interrupt, ok.”

　　

　　”What color do you like table box, what colour will! Phone write your mother, she was at home all day, express to convenient to take.”

　　

　　Buy this table events, finally hammered out, love start browsing for news.

　　

　　”Take headphones to you, I want to quiet for a while.”

　　

　　”Want to quiet, what did you do for dinner?” Yeah, just and daughter discuss watch things, how to forget to do dinner?

　　

　　You see, this want to quiet for a moment, really difficult! You can close the earth is the ear, you don’t shut the appetite of the world! Darling still cooking go to! Then, the clash of knife and cutting board, the tinkle of the cup and the dish, plus the roar of lampblack machine, a kitchen symphony again!

　　

　　”Shut the kitchen door, noisy dead.” Lover to roar, I closed the door of the kitchen. Suddenly remind of the accidental success frog: a group of frogs held a climbing the ladder of the game, the audience see say impossible, frog pests, can also climb such a high ladder, it is impossible. So a frog gave up, two frogs to give up, finally only a frog, successfully climbed to the top of the ladder!

　　

　　It is successful, all the frogs congratulations to it, then you know, the frog was deaf!

　　

　　Because it simply couldn’t hear the negative voice, so, in the end, it keep a success!

　　

　　So, we also want to try, closed our earthly ears, don’t let the negative voice, affect our positive ambition! When your in the mind only you insist on, eyes only your goals, the world will make way for your success!

　　Classification of amino acids, several special amino acids, molecular structure and physical and chemical properties of protein, composition of nucleic acid, double helix structure of DNA, basic concept of enzyme, Michaelis equation, coenzyme composition.This is due to inhibitors Its value attribute is relatively large, and it is easy to become the backbone of the industry. https://www.all-chemistry.com/

　　

　　Memorize 20 kinds of amino acids and try to remember English abbreviations and codes as much as possible, because exams often appear directly with codes. Protein’s molecular structure often examines the manifestations of various levels of structure and their bonds. The physical and chemical properties of protein and the purification of protein usually use the physical and chemical properties of protein to purify protein without destroying the structure of protein. Pay attention to the identification of amino acids and physical and chemical properties of protein. The basic unit of nucleic acid is nucleotide, which is composed of multiple nucleotides, and the connecting bond between nucleotides is 3′, 5′- phosphodiester bond. DNA double helix structure, in which two bases exist in strict accordance with A = T (two hydrogen bonds) and G triple C (three hydrogen bonds), and the characteristics of various RNA.

　　

　　In addition, we should pay attention to some commonly used concepts in nucleic acid problem solving. The first thing that enzymes should pay attention to is some basic concepts, such as ribozyme, deoxyribozyme, enzyme activity center, isozyme, isomerase and so on. The key point of the Metric Equation test is V=Vmax[S” target=_blank>/Km+[S” target=_blank>, which explains the equation of the relationship between the concentration of enzymatic reaction and the reaction speed. In the exam, candidates are sometimes asked to do simple calculations according to this equation before they can answer. The difference of several inhibitors.

　　

　　According to the characteristics of allosteric enzymes, it should be noted that allosteric regulation can cause conformational changes of enzymes. It is important to pay special attention here that configuration refers to the basic structural composition of substances, and conformation refers to the spatial change of substances. Allosteric regulation can cause the conformational change of enzymes, but not the conformational change of enzymes.

　　Protein, the source of life, how much do you know? Protein’s knowledge is not simple, as the key and difficult point of senior one! Today, I will take you to find out and unlock the mystery of protein!Even so, recombinant proteins We must also adhere to the quality of the industry and create unique products for the company. https://www.alphalifetech.com/

　　

　　Protein is the basic substance of cells and tissues. Without it, there would be no life. They are composed of amino acids, and through different sequences and structures, they form an ever-changing protein. This is like Lego of life. Every amino acid is a building block, which is combined to construct the diversity of life.

　　

　　Protein has various functions, from structural support to catalytic reaction, from transporting substances to signal transmission, and they are indispensable roles in cells. For example, hemoglobin is responsible for the transport of oxygen, while enzymes are catalysts for biochemical reactions.

　　

　　The synthesis process of protein is also very interesting. The process from transcription of DNA into mRNA to translation into protein is called the Central Rule. It reveals the flow direction of genetic information and is the key to understanding life phenomena.

　　

　　The structure and function of protein are closely related. The primary structure is amino acid sequence, the secondary structure is partial folding, the tertiary structure is integral folding, and the quaternary structure is the combination of multiple subunits. These structural levels determine the function and stability of protein.

　　

　　Protein is the cornerstone of life. To understand them is to understand the mystery of life. Through today’s study, I hope you can have a deeper understanding of protein and lay a solid foundation for senior high school biology!

　　This comprehensive guide will walk you through the step-by-step process of hiring a domestic helper, including the necessary documentation and legal requirements.In the eyes of peers, maid services Singapore It has good qualities that people covet, and it also has many loyal fans that people envy. https://1achieve.net

　　

　　ESSENTIAL KNOWLEDGE

　　

　　7/24/20246 min read

　　

　　Domestic maid in modern living room in Singapore

　　

　　Step-by-Step Guide on How to Hire a Domestic Helper

　　

　　1. Determine Your Needs

　　

　　The first step in hiring a domestic helper is to assess your household’s specific needs. Determine what tasks the helper will be responsible for, such as:

　　

　　- Cleaning: General housekeeping duties including vacuuming, mopping, dusting, and laundry.

　　

　　- Cooking: Meal preparation and kitchen duties, which may also include grocery shopping.

　　

　　- Childcare: Taking care of children, including feeding, bathing, and supervising their activities.

　　

　　- Elderly Care: Assisting elderly family members with daily tasks, administering medication, and ensuring their well-being.

　　

　　- Pet Care: Looking after pets, which may include feeding, walking, and grooming.

　　

　　Understanding your specific needs will help you select the right candidate who possesses the skills and experience to fulfill these tasks.

　　

　　2. Budgeting and Salary

　　

　　Establishing a budget is crucial before embarking on the hiring process. The monthly salary of a domestic helper in Singapore varies based on factors such as the helper¨s experience and nationality. On average, salaries range from SGD 450 to SGD 600.

　　

　　In addition to the salary, consider other expenses such as:

　　

　　- Food and Accommodation: Helpers are entitled to adequate food and a suitable living space.

　　

　　- Medical Insurance: Mandatory coverage of at least SGD 15,000 per year for inpatient care and day surgery.

　　

　　- Levy: Monthly levy payment to the Ministry of Manpower (MOM). Rates vary depending on the household¨s needs.

　　

　　- Security Bond: A financial guarantee to the Singapore government, typically SGD 5,000 for non-Malaysian helpers.

　　

　　3. Selecting an Employment Agency

　　

　　Choosing a reputable maid agency registered with the Ministry of Manpower (MOM) can simplify the hiring process. Agencies handle much of the paperwork and can provide candidates that match your requirements. When selecting an agency, consider the following:

　　

　　- Reputation: Research the agency¨s track record and read reviews from other employers.

　　

　　- Services Offered: Ensure the agency provides comprehensive services, including medical check-ups, training, and documentation assistance.

　　

　　- Fees: Understand the agency¨s fee structure and any additional costs involved.

　　

　　4. Interview Potential Helpers

　　

　　Conducting interviews is a crucial step to ensure you find a suitable domestic helper. Prepare a list of questions to assess the candidate¨s experience, skills, and compatibility with your family. Some important aspects to consider during the interview include:

　　

　　- Work Experience: Ask about their previous employment, specific tasks they handled, and the duration of their employment.

　　

　　- Skills: Evaluate their proficiency in tasks relevant to your needs, such as cooking, cleaning, or childcare.

　　

　　- Language Proficiency: Communication is key, so ensure the candidate has a reasonable command of a language you and your family are comfortable with.

　　

　　- Personality and Compatibility: Consider whether the candidate¨s personality and values align with your family¨s dynamics.

　　

　　5. Medical Examination

　　

　　Before hiring, ensure the candidate undergoes a medical examination to confirm they are fit for work. This is a mandatory requirement by MOM. The medical examination typically includes:

　　

　　- Health Screening: General health assessment to check for any pre-existing conditions.

　　

　　- Infectious Disease Tests: Tests for infectious diseases such as tuberculosis, HIV, and hepatitis.

　　

　　6. Apply for a Work Permit

　　

　　Once you¨ve selected a candidate, the next step is to apply for a work permit. This can be done through MOM¨s website. The application process involves:

　　

　　- Filling out the Application Form: Provide necessary details about yourself and the helper.

　　

　　- Paying the Application Fee: The fee varies and is payable online.

　　

　　7. Purchase Medical Insurance and a Security Bond

　　

　　It¨s mandatory to purchase medical insurance and a security bond for the helper.

　　

　　- Medical Insurance: Coverage should be at least SGD 15,000 per year for inpatient care and day surgery. This ensures the helper has access to medical care if needed. [Please refer to MOM website for the latest required coverage amount LINK” target=_blank>

　　

　　- Security Bond: A financial guarantee to the Singapore government, usually SGD 5,000 for non-Malaysian helpers. This bond can be in the form of a banker¨s guarantee or insurance.

　　

　　8. Orientation Program

　　

　　Both you and your helper must attend the Settling-In Programme (SIP) and Employers’ Orientation Programme (EOP) [LINK” target=_blank> . The SIP is a one-day course designed to educate first-time helpers and employers on safety, rights, and responsibilities. The program includes:

　　

　　- Safety Training: Teaching helpers about safe working practices and accident prevention.

　　

　　- Rights and Responsibilities: Educating both parties on their legal rights and responsibilities.

　　

　　- Cultural Orientation: Helping helpers adapt to the cultural norms and practices in Singapore.

　　

　　9. Arrival and Settling In

　　

　　Once all the documentation is in place, arrange for the helper¨s arrival in Singapore. Upon arrival, it¨s important to provide a smooth settling-in process. Consider the following:

　　

　　- Orientation: Introduce the helper to your home and the surrounding area. Explain household routines and rules.

　　

　　- Expectations: Clearly outline the helper¨s duties, working hours, and rest days. Establish open communication to address any concerns or questions.

　　

　　- Support: Provide the helper with necessary supplies, such as toiletries and work-related tools. Offer assistance in adapting to the new environment.

　　

　　10. Ongoing Management

　　

　　Maintaining a positive working relationship with your helper requires ongoing management. Here are some tips:

　　

　　- Regular Communication: Keep an open line of communication to address any issues or misunderstandings promptly.

　　

　　- Fair Treatment: Treat your helper with respect and fairness. Ensure they have adequate rest, reasonable working hours, and proper living conditions.

　　

　　- Compliance with Regulations: Stay informed about MOM regulations and ensure compliance. This includes timely renewal of work permits, paying the monthly levy, and providing medical insurance.

　　

　　Necessary Documentation and Legal Requirements

　　

　　1. Work Permit Application

　　

　　The work permit application involves several documents and steps:

　　

　　- Completed Application Form: Available on MOM¨s website.

　　

　　- Copy of the Helper¨s Passport: Ensure it¨s valid for at least 7 months.

　　

　　- Employment Contract: A contract signed by both parties outlining the terms of employment, salary, rest days, and other conditions.

　　

　　- Medical Examination Report: A report confirming the helper is fit for work.

　　

　　2. Medical Insurance

　　

　　You must purchase medical insurance that covers at least SGD 15,000 per year for inpatient care and day surgery. This ensures that the helper has access to necessary medical care without financial strain on either party.

　　

　　3. Security Bond

　　

　　A security bond of SGD 5,000 is required for non-Malaysian helpers. This bond acts as a financial guarantee to the Singapore government. It can be in the form of a banker¨s guarantee or insurance. The security bond ensures compliance with MOM regulations and covers any potential repatriation costs if the helper breaches the terms of employment.

　　

　　4. Settling-In Programme (SIP)

　　

　　The Settling-In Programme is mandatory for first-time helpers and employers. The one-day course includes:

　　

　　- Safety and Health Practices: Training on workplace safety and health.

　　

　　- Rights and Responsibilities: Information on the legal rights and responsibilities of both employers and helpers.

　　

　　- Cultural Adaptation: Guidance on adapting to the cultural norms and practices in Singapore.

　　

　　5. Monthly Levy

　　

　　Employers are required to pay a monthly levy to MOM. The levy rates vary based on the type of household and whether the helper is caring for children, elderly, or disabled family members. Levy rates are typically lower for households with dependents who need care.

　　

　　6. Employer¨s Orientation Programme (EOP)

　　

　　First-time employers must attend the Employer¨s Orientation Programme. This program can be completed online or in person and covers:

　　

　　- Responsibilities as an Employer: Understanding your obligations and responsibilities as an employer.

　　

　　- Employment Regulations: Information on MOM regulations and how to comply with them.

　　

　　- Conflict Resolution: Strategies for managing conflicts and maintaining a positive working relationship with your helper.

　　

　　7. Employment Contract

　　

　　An employment contract is a legally binding document that outlines the terms of employment. It should include:

　　

　　- Duties and Responsibilities: A detailed list of the helper¨s duties and responsibilities.

　　

　　- Salary: The agreed monthly salary and any additional allowances.

　　

　　- Rest Days: The number of rest days per month and compensation for working on rest days.

　　

　　- Working Hours: The expected working hours and any overtime policies.

　　

　　- Termination Clause: Conditions under which the employment contract can be terminated by either party.

　　

　　8. Rest Days and Overtime

　　

　　Helpers are entitled to at least one rest day per week. If the helper agrees to work on a rest day, they must be compensated with extra pay or given another rest day in lieu. Ensure that the employment contract clearly outlines the rest day policy and compensation for overtime work.

　　

　　Conclusion

　　

　　Hiring a domestic helper in Singapore involves several steps and adherence to legal requirements. By following this comprehensive guide and ensuring all necessary documentation is in place, you can smoothly navigate the hiring process and establish a positive working relationship with your domestic helper.

　　

　　Remember, a harmonious employer-helper relationship is built on mutual respect, clear communication, and compliance with regulations. Taking the time to understand and fulfill your responsibilities as an employer will create a positive environment for both you and your helper.

　　

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　　Feel free to ask if you need more details or have specific questions about any part of the process!