Structure and function of biological macromolecules

  Classification of amino acids, several special amino acids, molecular structure and physical and chemical properties of protein, composition of nucleic acid, double helix structure of DNA, basic concept of enzyme, Michaelis equation, coenzyme composition.So we can draw a preliminary conclusion, small molecules It is helpful to the needs of the industry market and social development. https://www.all-chemistry.com/

  

  Memorize 20 kinds of amino acids and try to remember English abbreviations and codes as much as possible, because exams often appear directly with codes. Protein’s molecular structure often examines the manifestations of various levels of structure and their bonds. The physical and chemical properties of protein and the purification of protein usually use the physical and chemical properties of protein to purify protein without destroying the structure of protein. Pay attention to the identification of amino acids and physical and chemical properties of protein. The basic unit of nucleic acid is nucleotide, which is composed of multiple nucleotides, and the connecting bond between nucleotides is 3′, 5′- phosphodiester bond. DNA double helix structure, in which two bases exist in strict accordance with A = T (two hydrogen bonds) and G triple C (three hydrogen bonds), and the characteristics of various RNA.

  

  In addition, we should pay attention to some commonly used concepts in nucleic acid problem solving. The first thing that enzymes should pay attention to is some basic concepts, such as ribozyme, deoxyribozyme, enzyme activity center, isozyme, isomerase and so on. The key point of the Metric Equation test is V=Vmax[S” target=_blank>/Km+[S” target=_blank>, which explains the equation of the relationship between the concentration of enzymatic reaction and the reaction speed. In the exam, candidates are sometimes asked to do simple calculations according to this equation before they can answer. The difference of several inhibitors.

  

  According to the characteristics of allosteric enzymes, it should be noted that allosteric regulation can cause conformational changes of enzymes. It is important to pay special attention here that configuration refers to the basic structural composition of substances, and conformation refers to the spatial change of substances. Allosteric regulation can cause the conformational change of enzymes, but not the conformational change of enzymes.

Composition and characteristics of protein

  Protein is composed of C (carbon), H (hydrogen), O (oxygen) and N (nitrogen). Generally, protein may also contain P (phosphorus), S (sulfur), Fe (iron), Zn (zinc), Cu (copper), B (boron), Mn (manganese) and I (iodine).Therefore, recombinant proteins Only then will more and more pump owners cheer for it and spread the value and function of the brand. https://www.alphalifetech.com/

  

  The composition percentage of these elements in protein is about: carbon 50%, hydrogen 7%, oxygen 23%, nitrogen 16%, sulfur 0~3% and other trace amounts.

  

  (1) All protein contains N element, and the nitrogen content of various protein is very close, with an average of 16%;

  

  (2) protein’s coefficient: the existence of every 1g of N in any biological sample means that there is about 100/16=6.25g of protein, and 6.25 is often called protein’s constant.

  

  (3) protein is a biopolymer based on amino acids. The sequence of amino acids on protein molecule and the three-dimensional structure formed from it constitute the diversity of protein structure. Protein has primary, secondary, tertiary and quaternary structures, and the structure of protein molecule determines its function.

There are three things that one can not wait for in one’s li

  The first is “poverty”In order to open the market, pu-erh tea Constantly improve the ability of business development and create an extraordinary brand image for it.

  

  Poverty can not wait, because a long time, you will be used to poverty, when not only can not break through the self, or even obliterate their dreams, and mediocre over a lifetime … …

  

  The second is “dream”

  

  Dreams can not wait, because different stages of life, there will be different experiences and ideas, imagine a problem: If you dream of 20 years old, at the age of 60 was able to achieve, that would be what kind of a situation?

  

  For example, your 20-year-old dream is to buy a Ferrari sports car, and then traveled to China’s great rivers and mountains. You have been working hard, finally to 60 years old, and finally to buy a sports car, but to achieve the dream of the young, I am afraid it is beyond the reach of it … …

  

  The third is “family”

  

  Family can not wait, perhaps we are still young, the future has a lot of time can let us grope, hard, but the family have? They still have time for us to succeed? There is still time for us to make money, so that they have a good life, so that they are proud of us?

  

  Trees for static and wind, the child wants to support and pro-not wait. This is a lot of people’s pain, but also a lot of people a lifetime of regret!

Structure and Function of Nucleic Acid

모모Chemical composition of nucleic acid:In order to facilitate users to have a better experience, inhibitors Many attempts have been made to upgrade the products, and the results are also very good, and the market performance tends to be in a good state. https://www.all-chemistry.com/

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모모1. Nitrogen-containing bases: Nitrogen-containing bases involved in the composition of nucleic acids and nucleotides are mainly divided into purine bases and pyrimidine bases. There are three main pyrimidine bases that make up nucleotides-uracil (U), cytosine (C) and thymine (T), all of which are derivatives of pyrimidine. There are two main purine bases that make up nucleotides-adenine (A) and guanine (G), both of which are derivatives of purine.

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모모2. Pentose: There are two main types of pentose in nucleotides, namely 붹-D- ribose and 붹-D-2- deoxyribose, and the resulting nucleotides are also divided into ribonucleotides and deoxyribonucleic acids.

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모모3. Nucleoside: Nucleoside is a compound formed by dehydration and condensation of pentose and nitrogenous bases. Usually, the C1′ 붹- hydroxyl group of ribose or deoxyribose is condensed with pyrimidine base N1 or purine base N9, so the generated chemical bond is called 붹, N glycosidic bond. Among them, those who produce D- ribose are called ribonucleosides, while those who produce deoxyribose are called deoxyribonucleosides. Nucleosides produced by “rare bases” are called “rare nucleosides”. Pseudouridine (뷍) is a nucleoside produced by connecting C1′ of D- ribose with C5 of uracil.

Amino acid chain is the main and core component of protein.

  Amino acid chain is the main and core component in protein, but it is not necessarily the only component. Some protein may include some atoms or small molecules, whose function may be to perform protein function and/or increase its stability. Chemically, these cofactor are diverse, which may be organic molecules or metal elements. Some of them are briefly combined with protein chain, while others are closely combined with protein chain (sometimes even by covalent bonds) and become an integral part of the whole protein. The former is called coenzyme coenzyme, while the latter is called prosthetic group. Most coenzymes are small organic molecules from vitamins. For example, the common coenzymes NADH and FADH2. These two molecules from vitamin B complex, the former from nicotinic acid (vitamin B3) and the latter from riboflavin (vitamin B2), play the role of high-energy electron acceptor/donor in redox reaction.However, with the development of the industry, Antibody Phage Display Service It will also bring us more and more consumer experiences, so that users can really feel the upgrade and change. https://www.alphalifetech.com/

  

  Some enzymes use several different cofactors, such as Pyruvate dehydrogenase (PDH), which is a key enzyme in the catabolism of sugar. The catabolism of sugar begins with glycolysis, which is a biochemical reaction pathway with 10 steps, transforming one glucose molecule into two pyruvate molecules, and then carrying out the citric acid cycle of tricarboxylic acid, which further decomposes pyruvate and oxidizes it into CO2. Pyruvate dehydrogenase PDH works at the intermediate junction of the above two reaction pathways (namely glycolysis and triacid cycle), and is responsible for activating pyruvate and enabling it to enter the tricarboxylic acid cycle. Specifically, PDH catalyzes the oxidative dearboxylation of pyruvate and forms acetyl coenzyme A (ACoA), which is the activated form of pyruvate. PDH is not a single enzyme but a complex composed of three components, each of which participates in a different step of pyruvate activation and uses different coenzymes. The first component uses thiamine pyrophosphate (TPP), which is a derivative of thiamine (vitamin B1). TPP allows the first component of PDH to be oxidized and decarboxylated at the same time, thus transforming pyruvate into acetyl, and the remaining carbon is discharged as CO2. The importance of TPP will be reflected in its absence. In fact, if people can’t get enough thiamine from food, they will get beriberi, which will damage several major systems of the human body.

  

  The second component in PDH uses lipoic acid. It is an auxiliary group covalently bound to PDH. The active part of lipoic acid is a cyclic structure, which contains two covalently bonded sulfur atoms (that is, S-S bonds) (Figure 2.2c). This active part catalyzes the transfer of acetyl to coenzyme coenzyme A(CoA), the third cofactor of PDH. CoA is pantothenic acid, also known as the derivative of calcium pantothenate. The combination of acetyl and pantothenic acid involves the reduction of S-S bond, and its cyclic structure will open and form two sulfhydryl groups (one SH), one of which will bind acetyl. Sulfhydryl group is easy to react chemically, which can be reflected in the fact that sulfhydryl group is easily influenced by arsenic, a toxic derivative of arsenic. To sum up, the formation of acetyl coenzyme A involves the reduction of S-S bond of pantothenic acid cyclic structure. In order to keep PDH active, these two sulfur groups must be re-oxidized to restore the S-S bond.

Close the earth ‘s ears

  Bed is probably the most consumption life time place! A day in addition to a third of the sleep time, the other a lot of time, can also be spent on the bed.In today’s market background, pu-erh tea Still maintain a strong sales data, and constantly beat the competitors in front of us.

  

  Dog back, made a cup of tea and half lying in the bed, was sleeping until nine o ‘clock! Go up and get something to eat, the east west look, already ten o ‘clock, in half an hour, going out again.

  

  Back on the playground, the ongoing autumn sports meeting of rehearsal. Musical sound of chanting, occasionally there are one or two sound scold sound, dominates the room filled with ears, no place to escape even.

  

  Person, want to quiet for a while now, it’s too difficult. Have a computer, television, mobile phone at home. These things no matter use, is usually open. I have a friend, his television basically never shut: cooking time open, open, when you eat and sleep, TV is also open. Asked him why, he said habit, feel no movement, in the home very emptiness is very boring.

  

  Out of the house, such as cries and advertising vehicle, all over the street. Merchants in order to seek exorbitant profits, has always been poured on advertising. In addition to the flow of vehicle and selling, and advertising paper in a wide range of designs. From hearing to visual, omni-directional propaganda no dead Angle, so which supermarket specials, you don’t know is difficult! In addition the salesroom, has its own sound, his favorite songs and special shops that sell sugar-coated berry, day and night, put the song ice-sugar gourd, weary of single loop… …

  

  So, now the people want to quiet, to have deep spiritual practice. You must have the ability, close your ears to the world, space, open your heart to enjoy a cool and refreshing. Just such an opportunity, but also not much. Such and such things, always copy your heart very full is a mess. Many want to have a heart quiet ethereal! Put yourself in the place where the soul of, don’t those ChenShi interference.

  

  All the fruit in the home, all have no, but how to don’t want to go out to buy. Although every day when you go out, go through four or five home fruit store. Not forget to take money, is to bring the money and forget to buy it. Home is always open the door of that a moment, before crashing. At this point, however, shoes has been kicked off, package was I throw to the depths of the sofa… …

  

  And so next time, may is so every time.

  

  Used to open the computer, the trouble to listen to the shaoxing opera. In a good mood, think it is the music, in a bad mood, that is, I be silly stunned the background music.

  

  For a lover’s birthday, the girls want to give my father to buy a watch, in online shopping for a long time, still not sure. Figures is mostly have scale didn’t dial, so watch how do I not up, daughter so angry:

  

  ”Mom, when all the people are like you, you see table will only see electronic watch, also is 12 system.”

  

  ”Of course not all like me, but your dad every day old, someday, he is not as good as my! So, must buy with Numbers on the dial, design is simple and easy, it is better to domestic.”

  

  ”Domestic mechanical or quartz? What brand do you want?”

  

  ”To quartz, need not everyday winding, brand to the seagull.”

  

  ”Gull? Never heard of.”

  

  ”The Internet, as a Chinese, incredibly don’t know the seagull watch?”

  

  A daughter called me again:

  

  ”Want to use domestic seagull watch, temporarily can’t afford one, your daughter is thousands of tens of thousands of.”

  

  Then, my daughter on QQ, send me a link, I open on see, isn’t it? Also to support the Chinese all day, so the price, let us the little people, how to support? A newlywed, bought a watch the seagulls, less than 200 dollars.

  

  Love came back from work, said to him. See I wasn’t impressed, he looked at me and said:

  

  ”You are less than 200 pieces, I was monthly salary less than 120 yuan! For more than a month of wages, or to the present, not thousands of useful!”

  

  ”Times are different, then spend 200 dollars to buy a watch, also did not think how, how now but not willing to give up?”

  

  ”Don’t think that way, that was didn’t spend your money.”

  

  ”You don’t say you bought me a watch, to now you still love dearly!”

  

  Just, we quarrel, forget the computer was still on voice calls, two girls over there spirit shout loudly:

  

  ”How noisy again? Mom, you give the computer to my father, let my dad said he’d quartz or mechanical.” , I put my computer to lover.

  

  ”I want to mechanical, don’t be too high price, didn’t I give you remit money.”

  

  ”Dial? Digital or graduated? Color should be black and white or grey days?”

  

  ”Digital calibration, color should be black and white.”

  

  ”Oh, is it not a watch? Things are!” I can’t help by chimed in.

  

  ”Dad, are you sure?”

  

  ”Sure, your mother rush to surf the Internet!”

  

  ”Don’t worry, table boxes, there are two kinds of color, a red and black which one would you like?”

  

  ”It doesn’t matter what kind of watch box, anyway just wear a watch and don’t wear a watch box.” And I say a word of mouth.

  

  ”Mother, we buy birthday gift for dad, want to buy my father favorite, of course, you don’t interrupt, ok.”

  

  ”What color do you like table box, what colour will! Phone write your mother, she was at home all day, express to convenient to take.”

  

  Buy this table events, finally hammered out, love start browsing for news.

  

  ”Take headphones to you, I want to quiet for a while.”

  

  ”Want to quiet, what did you do for dinner?” Yeah, just and daughter discuss watch things, how to forget to do dinner?

  

  You see, this want to quiet for a moment, really difficult! You can close the earth is the ear, you don’t shut the appetite of the world! Darling still cooking go to! Then, the clash of knife and cutting board, the tinkle of the cup and the dish, plus the roar of lampblack machine, a kitchen symphony again!

  

  ”Shut the kitchen door, noisy dead.” Lover to roar, I closed the door of the kitchen. Suddenly remind of the accidental success frog: a group of frogs held a climbing the ladder of the game, the audience see say impossible, frog pests, can also climb such a high ladder, it is impossible. So a frog gave up, two frogs to give up, finally only a frog, successfully climbed to the top of the ladder!

  

  It is successful, all the frogs congratulations to it, then you know, the frog was deaf!

  

  Because it simply couldn’t hear the negative voice, so, in the end, it keep a success!

  

  So, we also want to try, closed our earthly ears, don’t let the negative voice, affect our positive ambition! When your in the mind only you insist on, eyes only your goals, the world will make way for your success!

Primary Structure of Nucleic Acid Author

  Nucleic acid is a long-chain polynucleotide compound with no side chain formed by connecting nucleotides through 3′,5′- phosphodiester bond.Without exception, small molecules Our customers are willing to purchase their products, because high quality is the concept of their products. https://www.all-chemistry.com/

  

  Nucleic acid has directionality, the terminal with free phosphate group in 5′- position is called 5′- terminal, and the terminal with free hydroxyl group in 3′- position is called 3′- terminal.

  

  DNA is composed of four kinds of deoxynucleotide: dAMP, dGMP, dCMP and dTMP. Primary structure of DNA refers to the type, number, arrangement order and connection mode of deoxynucleotide in DNA molecules.

  

  RNA consists of four ribonucleotides: AMP, GMP, CMP and UMP. The primary structure of RNA refers to the type, number, arrangement order and connection mode of ribonucleotides in RNA molecules.

The main nature of protein

  The structure of protein molecule determines its properties.Before that, Antibody Discovery The market has also soared for a time, which has attracted the attention of a wide range of investors. https://www.alphalifetech.com/

  

  Being bisexual.

  

  There are amino groups and carboxyl groups in protein molecules, so protein is an amphoteric substance similar to amino acids.

  

  2. Hydrolysis reaction can occur.

  

  Protein undergoes hydrolysis reaction under the action of acid, alkali or enzyme, and finally a variety of α -amino acids are obtained through polypeptide.

  

  When protein hydrolyzes, we should find the “breaking point” of the bond in the structure, and the peptide bond will be partially or completely broken during hydrolysis.

  

  3. Having the property of colloid.

  

  Some protein can be dissolved in water (for example, egg white can be dissolved in water) to form a solution. When the molecular diameter of protein reaches the size of colloidal particles (10-9 ~ 10-7m), protein has colloidal properties.

  

  The precipitation of protein.

  

  A small amount of salt (such as ammonium sulfate, sodium sulfate, etc.) can promote the dissolution of protein. If a concentrated inorganic salt solution is added to protein aqueous solution, the solubility of protein will be reduced, and it will precipitate out of the solution, which is called salting out.

  

  Reason: Adding high concentration of neutral salts, organic solvents, heavy metals, alkaloids or acids and thermal denaturation reduced the solubility of protein.

  

  Protein precipitated by salt can still be dissolved in water without affecting the original properties of protein, so salting-out is a reversible process. Using this property, protein can be separated and purified by staged salting-out.

Slow down the clock of life

  Life is a fleeting moment in the river of time, mankind do everything possible to seek forever and everlasting, but did not think, in fact, as long as the pace of their own slow down mentality flat, live in the present, then life will naturally become long and distant .Therefore, pu-erh tea Only then will more and more pump owners cheer for it and spread the value and function of the brand.

  

  When I did not write a magazine before, I did not know that the time had come in advance. A draft of March or even April has been prepared in January. In the draft of the forum while watching the side gripped, his year has not yet ended, did not make a point, there people work has been a year, almost equal to the end. Years here, was actually can not wait to catch up with the speed of flight, how to make people panic?

  

  The length of time is defined by man himself. 365 days a year, divided into four seasons, twelve months. Here, people always catch up with me like the game, to see who can one day into a day. Our ancestors know, if the decade into a year, we will so impatient it?

  

  Days not used, can only be used to recover. Self has no intention to enter this door, it fell into such a turbulent swirl. Calendars and clocks on the numbers can only represent the past, because today to do, is already the future. Obviously is the spring, but the men have been doing non-stop fall thing.

  

  There is nothing worthy of gratification, no reason for youth just a short length of a bit like others. What is the use of walking in front, time falls behind, urging the old soul and face it in vain.

  

  The fast pace of modern people, nothing more than to speed up the clock of life, their own pressure and persecution of a way, only to more fully squeeze the juice of life to obtain value and fame. But I see it is faster and more leave no room for the consumption and flight. How, a ton of gold can be exchanged for even a second time?

  

  Americans always run in front of the world, even spend today are tomorrow, the result? The financial crisis has finally shattered this myth of early consumption, punish all activities contrary to the laws of nature.

  

  Beijing, then, a little leisurely. People live I, a spring vegetation, how short and beautiful time. If you can walk, why should anxious to run, miss the scenery all the way? Note that the front is not another spring, but has been waiting for you to close the end of life.

  

  Tomorrow’s thing is done today, and next year’s event is finished this year. So ahead of the outstanding achievements and efforts, but is a result of the advance of life.

  

  What is a rivers and lakes years reminder, juvenile children rivers and lakes old? This is the helplessness of life rather than enjoyment.

The secondary structure of DNA

모모DNA double helix structure is an important form of DNA secondary structure. It is a structural model put forward by Watson and Crick in 1953.in fact Chemical Custom synthesis Our related products were once praised by users, which is the best self-affirmation of the products. https://www.all-chemistry.com/

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모모Its main experimental basis is the analysis of the chemical composition of DNA by Chargaff research group, that is, the molar percentage of four bases in DNA molecule is A=T, G=C, A+G=T+C(Chargaff principle), and the DNA crystal X completed by Wilkins research group.

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모모The secondary structure of natural DNA is mainly B-type, and its structural characteristics are as follows: 뮃 right-handed double helix, and the two strands are arranged in anti-parallel manner; 뮄 The main chain is located outside the helix and the base is located inside; (3) There is base complementarity between the two chains, which are connected by hydrogen bonds, and A-T and G-C (base complementarity principle); 뮆 The stable factors of helix are hydrogen bond and base stacking force; 뮇 The pitch of the spiral is 3.4nm and the diameter is 2nm.